It has been proposed that cytokines may be involved in mediating the characteristic pathological changes in psoriasis, including epidermal hyperplasia, compromised keratinocyte differentiation, and dermal and epidermal infiltration by
polymorphonuclear
leukocytes and mononuclear cells.
The purpose of this study is to assess the pattern of cytokine gene expression in psoriatic skin lesion. To investigate the cytokine expression pattern, we examined the transcripts of cytokine genes using reverse transcription and polymerase
chain
reaction(RT-PCR) method with 13 cytokine-specific primers.
@ES The Results were as follow:
@EN 1. Only the TGF-βwere detected in both lesional and perilesional skin.
2. IL-1α, il-1β, Il-8, and TGF-β were detected in both lesional and perlesional psoriatic skin. The lesions showed more prominent expression.
3. IL-10 and GM-CSF were weakly detectable in three lesional psoriatic skin respectively, and IL-2R in two lesional psoriatic skin.
4. IL-2, Il-4, IL-6 TNF-α IFN-γwere not detectable in both lesional and perilesional psoriatic skin.
Therefore, differences in the cytokines gene expression were noted between normal and psoriatic skin. The patterns of cytokines gene expression in perilesional skin were similar to those of lesional psoriatic skin.
|